منابع مشابه
Multiple pathways contribute to nuclear import of core histones.
Nuclear import of the four core histones H2A, H2B, H3 and H4 is one of the main nuclear import activities during S-phase of the cell cycle. However, the molecular machinery facilitating nuclear import of core histones has not been elucidated. Here, we investigated the pathways by which histone import can occur. First, we show that core histone import can be competed by the BIB (beta-like import...
متن کاملStructure-based nuclear import mechanism of histones H3 and H4 mediated by Kap123
Kap123, a major karyopherin protein of budding yeast, recognizes the nuclear localization signals (NLSs) of cytoplasmic histones H3 and H4 and translocates them into the nucleus during DNA replication. Mechanistic questions include H3- and H4-NLS redundancy toward Kap123 and the role of the conserved diacetylation of cytoplasmic H4 (K5ac and K12ac) in Kap123-mediated histone nuclear translocati...
متن کاملThe requirement of H1 histones for a heterodimeric nuclear import receptor.
After synthesis in the cytoplasm, H1 histones are imported into the nucleus through an energy-dependent process that can be mediated by an importin beta-importin 7 (Impbeta-Imp7) heterodimer. H1 histones contain two structurally different types of nuclear localization signals (NLS). The first type of NLS resides within the unstructured C-terminal domain and is rich in basic amino acids. In cont...
متن کاملIn vitro nuclear import
T he nuclear pore serves as the gateway to the nucleus, allowing molecules to slip in and out. A technique devised by Larry Gerace (Scripps Research Institute in La Jolla, California) and colleagues helped researchers ferret out the biochemical details of nuclear import and export, identifying the driving force as the protein Ran. The same system helped scientists pinpoint the proteins that fer...
متن کاملNuclear Import of Yeast Proteasomes
Proteasomes are highly conserved protease complexes responsible for the degradation of aberrant and short-lived proteins. In highly proliferating yeast and mammalian cells, proteasomes are predominantly nuclear. During quiescence and cell cycle arrest, proteasomes accumulate in granules in close proximity to the nuclear envelope/ER. With prolonged quiescence in yeast, these proteasome granules ...
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ژورنال
عنوان ژورنال: Biochemical Society Transactions
سال: 2020
ISSN: 0300-5127,1470-8752
DOI: 10.1042/bst20200572